| CAO Jian_ping,SHENG Fang_jun,ZHU Wei.Study on effects of ATM gene on expression of hTERT in AT cells exposed to 60Co γ -rays[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):517-520 |
| Study on effects of ATM gene on expression of hTERT in AT cells exposed to 60Co γ -rays |
| Received:May 09, 2005 |
| DOI： |
| KeyWords:Ataxia-telangiectasia ATM Human telomerase reverse transcriptase Ionizing radiation |
| FundProject:国家自然科学基金资助项目(30170288);江苏省高校自然科学基金重点项目(04KJA180121);苏州大学江苏省级重点实验室开放经费资助项目(KJS05029);苏州大学医学发展基金重点项目(EE126032);江苏省研究生创新计划(Xm04-67) |
| Author Name | Affiliation | E-mail | | CAO Jian_ping | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | jpcao@suda.edu.cn | | SHENG Fang_jun | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | | ZHU Wei | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | | 罗加林 | 浙江省肿瘤医院 | | | 冯爽 | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215007, China | | | 樊赛军 | Department of Oncology， Lombardi Comprehensive Cancer Center，Georgetown University， Washington DC 20057， USA | | | F.Eckardt-Schupp | GSF-National Research Center Institute of Radiobiology(F.Eckardt-Schupp)； | |
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| Abstract:: |
| Objective To study the effects of exogenous ATM gene on mRNA and protein expression of hTERT (human telomerase reverse transcriptase, hTERT) of a fibroblast cell line (AT5BIVA cells,At cells for short) established from skin of the ataxia telangiectasia (AT) patients. Methods After the following cells had been exposed to 0, 1, 3, 5 Gy of 60Co γ-rays, RT-PCR and Western blotting were used to observe the mRNA and protein expressions of hTERT in AT,PEBS7(blank vector)-AT,ATM+(AT gene mutated)-AT and GM cells, respectively. The GM(GM0639) cells were used as the normal control in this experiment. Results Except for GM cells, there were mRNA and protein expressions of hTERT in all AT, PEBS7-AT and ATM++-AT cells before exposure to ionizing radiation. However, the mRNA and protein expressions of hTERT in ATM++-AT cells weve significantly lower than those in AT cells, but still higher than those in GM cells (P<0.05). Aftev exposure, the mRNA and protein expressions of hTERT in AT,PEBS7-AT,ATM++-AT and GM cells were increased dose-dependently from 1Gy to 5Gy. At the same dose point, the mRNA expression of hTERT in ATM++-AT cells was significantly lower than that of AT cells. Conclusion Exogenous ATM gene can down-regulate mRNA and protein expressions of hTERT in AT cells no matter wheter the latter have been exposed to ionizing radiation or not. The mRNA and protein expressions of hTERT in cells can be induced by ionizing radiation in a dose-dependent manner. Telomerase is speculated on to participate in the repair of DNA damaged induced by ionizing radiation. |
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