| YAN Feng-qin,WANG Jian-qiu,FU Shi-bo.Effects of ionizing radiation on the expression of P21 protein in EL-4 mouse lymphoma cells[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):514-516 |
| Effects of ionizing radiation on the expression of P21 protein in EL-4 mouse lymphoma cells |
| Received:March 07, 2005 |
| DOI： |
| KeyWords:Ionizing radiation EL-4 mouse lymphoma cells P21 Protein expression |
| FundProject:国家自然科学基金资助项目(30270346) |
| Author Name | Affiliation | E-mail | | YAN Feng-qin | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | juz@mail.jlu.edu.cn | | WANG Jian-qiu | 吉林大学再生医学科学研究所生物化学教研室 | | | FU Shi-bo | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 鞠桂芝 | MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To explore the effects of ionizing radiation on the expression of P21 protein. Methods Immunocytochemistry and flow cytometry were used to measure the changes in the expression of P21 protein in EL-4 cells along with the increment of time and radiation doses. Results After 4.0 Gy X-rays irradiation, marked elevation of P21 protein level was found in EL-4 cells at 2-72 h with immunocytochemical method and at 8-72 h with flow cytometry （P<0.01 or P<0.001, respectively）. On the other hand, it was observed that the level of P21 protein increased evidently with the delivery of 0.56.0 Gy X-rays by using immunocytochemical method and with the delivery of 1.04.0 Gy X-rays by using flow cytometry in EL-4 cells at 24 h after irradiation. Conclusion The P21 protein expression could be increased by X-irradiation in time-and dose-dependent manners. |
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