| XIANG Qing,XU Mei,LI Hong-yan.Study on melanoma cell lines mutated in outer space environment[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):501-504 |
| Study on melanoma cell lines mutated in outer space environment |
| Received:June 30, 2005 |
| DOI： |
| KeyWords:Outer space Melanoma B16 cell line Mutagenesis |
| FundProject:国家重点基础研究规划(“973”计划)前期资助项目(2003CA04200);国家自然科学基金资助项目(30371324) |
| Author Name | Affiliation | E-mail | | XIANG Qing | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | tjt@263.net | | XU Mei | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | LI Hong-yan | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 徐波 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 房青 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 郭宇鹏 | 昆明医学院第一附属医院胸外科 | | | 耿传营 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 潘琳 | 100029 北京, 中日友好医院临床医学研究所中心实验室 | | | 唐劲天 | 清华大学工程物理系医学物理与工程研究所 | |
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| Abstract:: |
| Objective To study the mutagenic effects of outer space environment on biological characteristics of melanoma B16 cell lines and screen the immunogenicity-enhanced cell line. Methods The murine melanoma B16 cells were carried in the No.20 return satellite and the survived cells were monocloned. One monoclonal cell line(1#), which morphology varied, was utilized for further study. MTT assay was used for analysis of cell proliferation. The tumor free time, immune organ weight and survival time were observed after subcutaneous or abdominal injection of the cells to C57BL/6J mice and the tumor lumps were examined by routine pathology. Results Compared with the control group, the morphology of 1# cell line showed dendritic form and its growth rate was insignificant as analyzed by SSPS. The tumor_free and survival times were both prolonged and the weights of tumors were reduced. The thymus gland coefficient was increased for 1# cell line. Simultaneously with that, the lymphocyte infiltration was augmented both in tumor and adjacent tissues in mice injected with 1# cells. Conclusions The 1# cell line,which was mutated in outer space environment, was trending towards differentiation. The present data imply that the immunogenicity of 1# cell line might be enhanced. Our observations could provide insights into the further screen of immunogenicity_enhanced cell lines. |
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