| LU Xue-chun,ZHU Hong-li,LOU Fang-ding.Protective effect of LRP16 over expression in HL-60 cell against DNA damage induced by ultraviolet[J].Chinese Journal of Radiological Medicine and Protection,2005,25(5):477-479 |
| Protective effect of LRP16 over expression in HL-60 cell against DNA damage induced by ultraviolet |
| Received:June 09, 2005 |
| DOI： |
| KeyWords:LRP16 gene DNA damage Single cell gel electrophoresis Violet |
| FundProject:国家973重点基金资助项目(2005CB522400);国家自然科学基金资助项目(39970318) |
| Author Name | Affiliation | E-mail | | LU Xue-chun | Geriatric Department of Hematology, PLA General Hospital of China, Beijing 100853, China | chunhuiliyu@yahoo.com | | ZHU Hong-li | Geriatric Department of Hematology, PLA General Hospital of China, Beijing 100853, China | | | LOU Fang-ding | Geriatric Department of Hematology, PLA General Hospital of China, Beijing 100853, China | | | 韩卫东 | Geriatric Department of Hematology, PLA General Hospital of China, Beijing 100853, China | | | 徐周敏 | Geriatric Department of Hematology, PLA General Hospital of China, Beijing 100853, China | | | 于力 | Geriatric Department of Hematology, PLA General Hospital of China, Beijing 100853, China | |
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| Abstract:: |
| Objective LRP16 gene was first cloned by our study group. Based on its theory protein sequence with a H2A1C like domain, We predicted its product has protective effect against DNA damage induced by ultraviolet and validated the effect by experiment. Methods LRP16 gene sequence was obtained from GeneBank, then was its theory amino acid sequence. After that, we search possible domain on this assumed protein. At the same time, we constructed a pcDNA3.1-LRP16 vector which could express LRP16 gene stably in cell lines. Then the vector was transfected into HL-60 cell by Superfect and the cell which had stably LRP16 gene expression were left by G418 selected. HL-60 cells were caused DNA damage by ultraviolet. Single cell gel electrophoresis(SCGE) was used to test the DNA damage and repair pattern difference among a pcDNA3.1-LRP16, a pcDNA3.1(+) and HL60 group. Results There is a domain something like human hismacro, COG2110 and A1pp from amino acid residue 148 to 315. LRP16 over expression in HL-60 cell had the protective effect against DNA damage caused by ultraviolet. Conclusion LRP16 protein may have protective effect against DNA damage induced by ultraviolet; |
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