| ZHANG Hui-lai,WANG Hua-qing,YAO Zhi,et al.Study on effect of ginsenoside Rg3 on immunological recovery after peripheral blood stem cell transplantation in animal experiments[J].Chinese Journal of Radiological Medicine and Protection,2005,25(5):431-434 |
| Study on effect of ginsenoside Rg3 on immunological recovery after peripheral blood stem cell transplantation in animal experiments |
| Received:March 29, 2005 |
| DOI： |
| KeyWords:Peripheral blood stem cell transplantation (PBSCT) Mice Immunological recovery Immune therapy |
| FundProject:天津市科委自然科学基金资助项目(003119711) |
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| Abstract:: |
| Objective To study the effect of ginsenoside Rg3 on immunological recovery in mice after peripheral blood stem cell transplantation (PBSCT). Methods A murine model of PBSCT was established. Ninety recipient mice were divided into 6 groups after transplantation: A, B, C, D, E, F groups, 15 mice each. They were intra-abdominally injected with Rg3 3 mg/kg, Rg3 6 mg/kg, Rg3 9 mg/kg,IL-2 and normal saline (NS), respectively. They were injected began from day 15 after PBSCT, daily for successive 15 dats per month, for a total of 3 months. The functional recovery of T and B lymphocytes was observed by the lymphocyte transformation test;and formation test of antibody, respectively, and the functional recovery of NK cell was observed by the killing test. The changes in number of T cell subpopulations, B cells and NK cells were observed by Flow cytometry (FCM). Results For the influence of Rg3 on cyto-immunity the action of 6 mg/kg and 9 mg/kg Rg3 was much better than that of NS (P<0.01) but not difference from that of IL-2. Only the combined group showed a good synergistic effect on immunological recovery. For the influence of Rg3 on humoral immunity, the effect of Rg3 3 mg/kg was stronger than NS but weaker than IL-2. The effect of Rg3 6 mg/kg and 9 mg/kg was similar to that of IL-2 (P>0.05). Only the combined group showed a good synergistic effect which was much better than that of other groups.For the influence on cytotoxic activity, the test groups were all better than NS group at the first month after PBSCT (P<0.05), but in the second month the cytotoxic activity of all test groups reduced gradually. However, in the third month the activity gradually recovered again, especially in Rg3 6 mg/kg, 9 mg/kg and 9 mg/kg+IL-2groups (P<0.01). Conclusion The combined use of ginsenoside Rg3 (high dose) and biological response modifier (BRM) improves greatly the immunological recovery of transplanted mice, and enhances greatly the immune function. The effect of combined therapy is much better than single IL-2 or Rg3 treatment. |
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