| HAO Yi-wen,LI Ya-ming,LIU Xian-zhi.Study on the relation among protein kinase C activity,adhesion molecules expression of irradiated vascular endothelial cells and transmigration of haemopoietic stem cells[J].Chinese Journal of Radiological Medicine and Protection,2005,25(5):428-431 |
| Study on the relation among protein kinase C activity,adhesion molecules expression of irradiated vascular endothelial cells and transmigration of haemopoietic stem cells |
| Received:October 16, 2004 |
| DOI： |
| KeyWords:γ-rays Vascular endothelial cells Potein kinase C Adhesion molecules Migration Haemopoietic stem cells |
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| Abstract:: |
| Objective To study the relation among protein kinase C (PKC),adhesion molecules expression of vascular endothelial cells irradiated by small dose of γ-rays and transmigration of haemopoietic stem cells. Methods Human umbilical vein endothelial cells (HUVECs) were irradiated with 1-5 Gy γ-rays.The PKC activity of irradiated cells was measured by the incorporation of [γ-32P] ATP into exogenous substrate.The expression of adhesion molecules VCAM-1,PECAM-1, CD44 in irradiated HUVECs was detected by ELISA and transmigration of CD34++ haemopoietic stem cells across the irradiated HUVECs was determined by the transwell migration assay. Results PKC in HUVECs was activated by less than 3 Gy γ-irradiation,resulting in membranous PKC activity increasing and cytosolic PKC activity decreasing gradually.The dominant change occurred in HUVECs irradiated by 2-3 Gy γ-rays (P<0.01).There was a decreasing trend of membranous and cytosolic activity PKC in HUVECs irradiated by more than 3 Gy γ-rays.The expressions of VACM-1,PECAM-1 and CD44 in HUVECs irradiated by less than 3 Gy γ-rays were significantly increased compared with the results of the control group(P<0.01).The numbers of CD34++ haemopoietic stem cells migrating across the irradiated HUVECs were much higher than those in the control group (P<0.01),but monoclonal antibodies to VACM-1,PEACM-1,CD44 could depress the transmigration activity.There was a positive relation beween the PKC activity and the expression of VCAM-1,PECAM-1 and CD44 respectively. Conclusions Optimal small dose of γ-rays could activate PKC,enhance PKC activity of irradiated vascular endothelial cells,and up-regulate the adhesion molecules expression of VACM-1,PECAM-1 and CD44,resulting in promoting the transendothelial migration of haemopoietic stem cells. |
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