| YANG Jian-she,LI Wen-jian,WANG Ju-feng.Kinetic repair of chromosome breaks in normal human liver cells irradiated with γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2005,25(5):423-425 |
| Kinetic repair of chromosome breaks in normal human liver cells irradiated with γ-rays |
| Received:December 17, 2004 |
| DOI： |
| KeyWords:Human Liver cells Chromosome break Kinetic repair γ-ray |
| FundProject:国家自然科学基金重点项目(10335050);科技部重大基础研究前期研究专项资助项目(2003CCB00200) |
| Author Name | Affiliation | | YANG Jian-she | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | LI Wen-jian | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | WANG Ju-feng | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 王转子 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 夏景光 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 金晓东 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 高清祥 | 兰州大学生命科学学院 |
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| Abstract:: |
| Objective To investigated the 48-hour kinetic repair of G2-phase normal human liver cells (cell line L02) exposed to γ-rays. Methods The chromosomal breaks were detected by premature chromosome condensation technique. Results The chromatid-type and isochromatid-type breaks increased with increase of the dose at 0 hour measured by PCC, the number of chromatid-type breaks was several times more than that of isochromatid-type breaks.Through further 24-hour incubation after exposure to radiation,both of these types of breaks were repaired to different extents,i.e., by 50% for chromatid-type breaks,15% for isochromatid-type ones at most.At 48th hour,there was a slight change of chromosome breaks compared with that of 24th hour (P>0.05). Conclusion The main type of chromosomal break is chromatid-type after exposure to γ-rays,and these bveaks are easy to be repaired. Though the isochromatid-type break is obviously less than that of the chromatid-type but is difficult to be repaired, indicating that the isochromatid type break is the important factor causing cell death and canceration when cells bave been exposed to γ-radiation. |
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