| PENG Rui-yun,GAO Ya-bing,CHEN Hao-yu.The change and significance of bFGF gene expression in the intestine of mice induced by neutron radiation[J].Chinese Journal of Radiological Medicine and Protection,2005,25(5):412-415 |
| The change and significance of bFGF gene expression in the intestine of mice induced by neutron radiation |
| Received:January 10, 2005 |
| DOI： |
| KeyWords:Neutron Intestines bFGF Immunohistochemistry In situ hybridization |
| FundProject:国家自然科学基金资助项目(30370438);全军“十五”指令性课题(01L019) |
| Author Name | Affiliation | | PENG Rui-yun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | GAO Ya-bing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | CHEN Hao-yu | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 王德文 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 付凯飞 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 吴小红 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 王水明 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 马俊杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 胡文华 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
|
| Hits: 2673 |
| Download times: 1700 |
| Abstract:: |
| Objective To study the change and significance of basic fibroblast growth factor (bFGF) gene expression in the intestine of mice induced by neutron radiation. Methods Altogether 230 male BALB/C mice were irradiated with neutron and sacrificed at 6 hours, 12 hours, 1, 2, 3, 4, 5, 7, 10, 14, 21 and 28 days respectively after irradiation. The protein and mRNA of bFGF were detected by means of immunohistochemistry and in situ hybridization. Results Neutron radiation caused apoptosis and necrosis of cryptal cells and these changes were related to the radiation dose. The intestinal mucosa was injured and then recovered obviously in 2.5 Gy neutron group, while after over 4.0 Gy irradiation no recovery was noted. The protein and mRNA of bFGF were positive in the plasma of epithelial cells and the mRNA was also strongly positive in the endothelial and intestitial cells in normal intestinal mucosa. The bFGF protein was decreased progressively within 3 days after 2.5 Gy neutron irradiation, then increased and peaked on day 5. Recovery to normal level was seen on day 14. The bFGF protein was progressively decreased within 4 days after neutron irradiation over 4.0 Gy. The mRNA of bFGF had the similar changes to its protein, except the peak of the mRNA was on day 3 and recovery on day 10. Conclusion Some doses of neutron radiation may induce intestinal injury which has relationship with the radiation dose. The expression of endogenous bFGF gene plays an important role in the course of damage and recovery of the intestine, and may accelerate its recovery. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
