XU Zhou-min,YU Li,LOU Fang-ding.Gene LRP15, a novel gene involved in repairing UV-induced DNA damage[J].Chinese Journal of Radiological Medicine and Protection,2005,25(4):398-400 |
Gene LRP15, a novel gene involved in repairing UV-induced DNA damage |
Received:August 12, 2004 |
DOI: |
KeyWords:LRP15 gene Eukaryotic expression DNA damage DNA repair |
FundProject:国家自然科学基金资助项目(3997082);全军医药卫生“十五”杰出人才基金资助项目(01J007) |
Author Name | Affiliation | XU Zhou-min | Department of Hematology, Shanghai Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China | YU Li | 解放军总医院血液科 | LOU Fang-ding | 解放军总医院血液科 | 卢学春 | 解放军总医院血液科 | 杨龙 | Department of Hematology, Shanghai Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China | 吕鸣 | Department of Hematology, Shanghai Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China | 吕有灵 | Department of Hematology, Shanghai Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China | 陈焱 | Department of Hematology, Shanghai Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China | 崔杰 | Department of Hematology, Shanghai Corps Hospital, Chinese People's Armed Police Forces, Shanghai 201103, China |
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Abstract:: |
Objective To explore the possible function of a novel gene LRP15 in repairing UV-induced DNA damage.Method Recombinant vectors containing the sense/antisense open reading frame (ORF) of LRP15 gene were constructed with a pcDNA3.1 eukaryotic expression vector. K562 cells were transfected with these two kinds of recombinant vectors by Superfect®. The transfected cells were chosen by geneticin (G418). The single cell gel electrophroesis (SCGE) assay was used to detect UV-induced DNA damage. Result The sense/antisense LRP15 recombinants were both successfully obtained with the molecular biological technique. The basal DNA damage of the experimental group was similar to that of the control group after UV exposure(P=0.156). But significant difference in DNA migration lengths was observed between the two groups at 45 min and 90 min after UV exposure(P<0.001). Conclusions The two kinds of eukaryotic expression vectors containing the sense/antisense LRP15 gene was successfully constructed. The study also suggests that the novel gene LRP15 may be a candidate for DNA repair gene playing an important role in repairing the UV-induced DNA damage. |
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