| TU Xu-min,LEI Su-wen,WANG Ju-fang.Study on bystander effect and associated mechanism mediated through culture medium[J].Chinese Journal of Radiological Medicine and Protection,2005,25(4):333-335 |
| Study on bystander effect and associated mechanism mediated through culture medium |
| Received:December 28, 2004 |
| DOI： |
| KeyWords:Activity of natural killer cells Bystander effect Reactive oxygen species Tumor cell Yac-Ⅰ |
| FundProject:国家自然科学基金资助项目(10105012) |
| Author Name | Affiliation | | TU Xu-min | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | LEI Su-wen | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | WANG Ju-fang | 中国科学院近代物理研究所 | | 张斌 | 西安市疾病预防控制中心 | | 张志兴 | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | 吕慧敏 | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | 李文建 | 中国科学院近代物理研究所 |
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| Abstract:: |
| Objective To study the bystander effect and associated mechanism mediated through the irradiated cell culture medium. Methods Splenic natural killer (NK) cells were obtained from healthy male ICR strain mice. Culture medium irradiated with different doses of 60Coγ-rays was used for culturing Yac-Ⅰ lymphoma cells. The degree of injury of the latter by activated NK cells was observed. A part of the culture media were pretreated with 1% DMSO, a scavenger of reactive oxygen species(ROS),in order to investigate the possible mechanism of a radiation-induced bystander response. Results Severer injury was induced in Yac-Ⅰ cells cultured in the media pre-irradiated with different doses of γ-rays than that in Yac-Ⅰ cells cultured in unirradiated medium, as shown by increased sensitivity to murine splenic NK cells (P<0.01). Culturing Yac-Ⅰ cells in DMSO-pretreated medium considerably reduced the activation of NK cells, especially in 0.25 Gy and 0.5 Gy γ-irradiated media. Therefore, it can be expected that DMSO can partly suppress ROS-induced bystander effect. Conclusion The irradiated culture medium of Yac-Ⅰ cells can trigger bystander effect. ROS likely plays an important role in radiation-induced bystander effect that can be partly suppressed by pretreatment with DMSO. |
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