| LI Yang,SONG Liang-wen.Effect of γ-rays on transcriptional activity of SP1, AP1 and Smad3-Smad4 in pulmonary fibroblasts[J].Chinese Journal of Radiological Medicine and Protection,2005,25(4):329-332,324 |
| Effect of γ-rays on transcriptional activity of SP1, AP1 and Smad3-Smad4 in pulmonary fibroblasts |
| Received:October 30, 2004 |
| DOI： |
| KeyWords:γ-rays Transcriptional factor Type Ⅰ collagen Lung fibroblast |
| FundProject:国家自然科学基金资助项目(30170292) |
| Author Name | Affiliation | | LI Yang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | SONG Liang-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 王德文 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 彭瑞云 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 高亚兵 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | 马俊杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To investigate the effects of γ rays on the transcriptional activity of SP1, AP1 and Smad3-Smad4 in pulmonary fibroblasts. Methods Rat pulmonary fibroblasts were separated,cultured and then irradiated with 3, 5 and 8 Gy 60Coγ-rays. The transcriptional activity of SP1, AP1 and Smad3-Smad4 and the expression of PAI-Ⅰ and type Ⅰcollagen were observed by means of EMSA, immunoblotting and immunohistochemistry. Results In normal fibroblasts, AP1, Smad3-Smad4 formed weak specific shift binding blot,but SP1 did not. After irradiated with 3, 5 and 8 Gy 60Coγ-rays, the transcriptional factors AP1, SP1 and Smad3-Smad4 were activated and the expression levels of type Ⅰcollagen and PAI-Ⅰ were increased. Conclusion A certain dose of γ-rays may promote the activation of transcriptional factors SP1, AP1 and Smad3-Smad4 and thus increase the expressions of PAI-Ⅰ and type Ⅰ collagen. |
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