| XIONG Jie,HAN Ling.Study on radiation carcinogenesis-related differentially expressive genes with suppressive subtractive hybridization[J].Chinese Journal of Radiological Medicine and Protection,2005,25(4):322-324 |
| Study on radiation carcinogenesis-related differentially expressive genes with suppressive subtractive hybridization |
| Received:December 01, 2004 |
| DOI： |
| KeyWords:Suppressive subtractive hybridization Radiation carcinogenesis Differentially expression cDNA library |
| FundProject:国家自然科学基金资助项目(30170282) |
| Author Name | Affiliation | E-mail | | XIONG Jie | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | linghan8888@yahoo.com.cn | | HAN Ling | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | | 朱丹 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | | 高建国 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | |
|
| Hits: 2378 |
| Download times: 1818 |
| Abstract:: |
| Objective To research differentially expressive genes related with radiation induced tumors in mice. Methods A mouse leukemia model was induced by 60Coγ-ray irradiation and the cDNA library of differentially expressive genes between normal thymus and thymus tumor was established with suppressive subtractive hybridization. Positive clones were identified and studied respectively by DNA sequencing. Results A total of 102 positive clones were screened out by suppressive subtractive hybridization. After PCR for 30 randomly selected clones, 28 clones showed anticipated inserted cDNA sequence. These cDNA sequences represented 24 known genes including three repeatedly detected genes comparing with GeneBank database. Conclusions The cDNA library of differentially expressive genes related to radiation carcinogenesis was successfully established and 24 ascendingly expressive genes were screened out. This study will contribute to enrich the molecular mechanism of radiation carcinogenesis. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
