| GUO Yu-peng,TANG Jin-tian,XU Mei.Oncogenesis of melanoma B16 cell clones mutagenized by space environment[J].Chinese Journal of Radiological Medicine and Protection,2005,25(4):301-304 |
| Oncogenesis of melanoma B16 cell clones mutagenized by space environment |
| Received:May 10, 2005 |
| DOI： |
| KeyWords:Extraterrestrial environment B16 cell Mutagenesis |
| FundProject:国家重点基础研究规划“973”计划前期资助项目(2003CA04200);国家自然科学基金资助项目(30371324) |
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| Abstract:: |
| Objective To explore the oncogenesis of the melanoma B16 cell clones mutagenized by space environment, and find the B16 cell clones with remarkably mutated immunogenicity. Methods B16 cells were carried by the Chinese 20th recoverable satellite to the outer space, and were harvested after 18 days' spaceflight and then monocloned. Four cell clones, which were randomly selected from the total 110 clones obtained, and the control clone were routinely cultured. The cultured cells were injected to 10 groups of C57BL/6J mice,8-11 mice in each group. Five groups of mice received hypodermic injection and another 5 groups of mice received abdominal injection. The survival time was observed in abdominal injection groups. The mice in hypodermic injection groups were sacrificed after 14 days, the tumor, spleen and thymus were weighted,and the serum IL-2 concentration was determined. Moreover, the melanoma tumor tissues were examined histopathologically. Results An experiment program suitable to screening space mutagenesis of B16 tumor cell clones in vivo and the observation indices were basically established. One clone was found out which was remarkably different from the control clone in latent period of tumor formation, tumor weight, survival time of the tumor-bearing mice and the expression of IL-2. Conclusions Cultured melanoma B16 cells could be mutated by outer space environment. The further study will be focused on the influence of space environment on immunogenicity of mutagenized B16 cells. |
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