| SUN Zu-yue,FU Jing-yi,LIU Jian-xiang.Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):248-251 |
| Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma |
| Received:April 12, 2004 |
| DOI： |
| KeyWords:Tumor-antigen peptide (TAP) T cell Anti-tumor effect Tumor immune Low dose radiation |
| FundProject: |
| Author Name | Affiliation | | SUN Zu-yue | Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China | | FU Jing-yi | Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China | | LIU Jian-xiang | 中国疾病预防控制中心辐射防护与核安全医学所 | | 付士波 | 吉林大学卫生部放射生物学重点实验室 | | 李修义 | 吉林大学卫生部放射生物学重点实验室 | | 刘树铮 | 吉林大学卫生部放射生物学重点实验室 | | 赵勇 | Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China | | 龚守良 | 吉林大学卫生部放射生物学重点实验室 |
|
| Hits: 2805 |
| Download times: 1677 |
| Abstract:: |
| Objective To determine whether there is synergically inhibitory effect of low dose radiation (LDR) and tumor-associated antigen peptides (TAP) on tumor growth in vivo, which may provide experimental basis for potential clinical co-application of these two approaches to treat cancers. Methods TAP extract (MW≤3×106) from tumor cell membrance was prepared with mild acid elution method, as reported. The mice were whole-bodily irradiated with 75 mGy X-rays 12 h before immunization with TAP extract. After immunization, the levels of CD3, CD69,TCRαβ cells and T cell subsets in the spleen were detected with FACS. The tumor growth rate was estimated, and the responses to Con A, the cytokine productions and CTL activities of splenocytes were also analyzed 7 d after immunization with TAP. Results The present experimental results showed that the TAP extract significantly reduced the incidence of the transplanted tumor, delayed the average appearing time and decreased the growth speed of the tumor. The response of splenocytes from mice immunized with TAP extract to Con A increased significantly compared with that in the control group.Irradiation with 75 mGy X-rays 12 h before immunization further enhanced the inhibitory effect of TAP extract on tumor growth, and increased the percentage of CD8+ splenocytes. Conclusion These results suggest that whole-body irradiation with LDR exerts a synergic inhibitory effect with TAP on tumor growth in vivo,in which enhanced cellular immune responses may be involved. ; |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
