| WAN Hong,ISHIHARA Hiroshi,TANAKA Izumi.Analysis of the upstream domain of radiation-induced junB gene[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):238-240 |
| Analysis of the upstream domain of radiation-induced junB gene |
| Received:January 07, 2005 |
| DOI： |
| KeyWords:junB promoter CAT reporter molecular Immediately-early response gene Signal transduction |
| FundProject:日本科学技术厅国际交流资助项目 |
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| Abstract:: |
| Objective To analyze the upstream domain of radiation-induced junB gene. Methods (Four plasmids) containing 250 bp, 590 bp, 900 bp, 1560 bp separately and CAT reporter gene were constructed and transferred to L8704 cells. The cells were incubated for different time intervals after 2 Gy X-irradiation. Total RNA was extracted from the cells and the fluctuation of CAT mRNA level was assessed by the RNA ratio of CAT/β-actin measured by quantitative Northern blot hybridization. Results CAT mRNA expression containing 900 bp and 1560 bp junB promoter was remarkably and rapidly increased, and reached its peak 30 min after 2 Gy X-irradiation. Conclusion The upstream domain of 590-900 bp junB gene is specifically radiosensitive and plays an important role in the early response of the cells to radiation. ; |
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