| WEI Qi-chun,SHEN Li,ZHU Yong-liang.Characterization of a radioresistant lung cancer cell subline D6-R[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):235-237 |
| Characterization of a radioresistant lung cancer cell subline D6-R |
| Received:October 22, 2004 |
| DOI： |
| KeyWords:Radiation Radiosensitivity DNA Damage DNA Repair Apoptosis |
| FundProject:浙江省医药卫生科研基金资助项目(2004A048);浙江省教育厅资助项目(20040207) |
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| Abstract:: |
| Objective To investigate the radioresistant mechanism of the newly established D6-R subline with respects to cell growth kinetics, radiation-induced apoptosis, DNA damage and repair. Methods D6-R and the parent D6 cells were engaged in this study. Cell growth kinetics was determined using MTT assay, apoptosis was analyzed by flow cytometry and fluorescence microscopy,and DNA damage and repair were detected using alkaline comet assay. Results D6-R and D6 cells showed the same growth kinetics pattern, and no evident apoptosis could be found pre- and post-irradiation in both cell lines. There were significant differences in radiation-induced initial DNA single strand breaks(SSB) and repair capacity between D6-R and D6 cells,i.e., D6-R showed less initial DNA SSB and better repair capacity. Conclusion The milder initial DNA damage and stronger repair capacity exhibited in D6-R cells might be the cause of their radioresistance. |
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