| ZHANG Jun-quan,GAO Rong-lian,CHEN Xiao-hua.Proteomic analysis of PC12 cell differentiation induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):228-230 |
| Proteomic analysis of PC12 cell differentiation induced by ionizing radiation |
| Received:January 10, 2005 |
| DOI： |
| KeyWords:Ionizing radiation PC12 cell Proteomics |
| FundProject:全军“十五”指令性基金课题(01L018) |
| Author Name | Affiliation | | ZHANG Jun-quan | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | GAO Rong-lian | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | CHEN Xiao-hua | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | 王治东 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | 董波 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | 饶亚岚 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | 侯丽莉 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | 张浩 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China | | 毛秉智 | Institute of Radiation Medicine, Academy of Military Medical Science, Beijing 100850, China |
|
| Hits: 2645 |
| Download times: 1790 |
| Abstract:: |
| Objective To explore the molecular mechanism of PC12 cell differentiation induced by ionizing radiation and screen the molecular target of nervous system injuried by irradiation. Methods PC12 cells were irradiated with 16 Gy 60Coγ ray. Total proteins of normal and irradiated cells were prepared 48 hours after irradiation and separated with two dimensional gel electrophoresis. Some differential expressed proteins were characterized with mass spectrometry. Results 876 differential expressed proteins were observed. Up-regulated expression of ubiquitin carboxyl-terminal hydralase L1 was found. Down-regulated expression of new protein similar to HP1α was found. Conclusion The characterization of some differential expressed proteins through proteomic analysis would benefit the research of molecular mechanism of PC12 cell differentiation induced by ionizing radiation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
