| DU Wei-xia,FU Ying-hua,CAO Jian-ping.Study on radiosensitivity and adaptive response of AT cells by using chromosome aberrations[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):225-227 |
| Study on radiosensitivity and adaptive response of AT cells by using chromosome aberrations |
| Received:February 16, 2005 |
| DOI： |
| KeyWords:Radiosensitivity Adaptive response AT cells Chromosome aberration |
| FundProject:国家自然科学基金资助项目(30170288) |
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| Abstract:: |
| Objective To explore radiosensitivity of AT (ataxia telangiectasia)cells and to find out whether cytogenetic adaptive response of AT cells could be induced by low-dose of ionizing radiation. Methods (AT5B1VA(AT) cells) and GM0639(GM)cells at G1 phase were exposed to a priming dose of 2 cGy 60Coγ- rays 6 hours before irradiation with a challenge dose of 1.0 Gy or 3.0 Gy 60Coγ-rays,and then chromosome aberrations were analyzed. Results The GM cells exposed to 2 cGy 60Coγ- rays could reduce the frequencies of chromosome aberrations induced by following irradiation with 1.0 Gy or 3.0 Gy 60Coγ- rays,i.e. the observation values for chromosome aberration were significantly lower than expectation values(P<0.001). However, for AT cells this phenomenon was not observed, i.e. the observation values for chromosome aberration were similar to expectation values(P>0.05). On the other hand, various frequencies of chromosome aberrations induced with 1.0 Gy or 3.0 Gy 60Coγ-rays for AT cells were significantly higher than those for GM cells(P<0.001). Conclusion For AT cells the cytogenetic adaptive response could not be induced with low dose of ionizing radiation, but the chromosome aberrations induced by ionizing radiation for the AT cells were highly sensitive for them. The reason might be due to that the AT cells could not correctly repair DNA double- strand break. |
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