| ZHAO Li-hua,LIU Bin,YU Xiao-fei.Protection from radiation injury through oral administration of PF4 gene carried by attenuated salmonella[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):217-220 |
| Protection from radiation injury through oral administration of PF4 gene carried by attenuated salmonella |
| Received:April 29, 2004 |
| DOI： |
| KeyWords:Attenuated salmonella Platelet factor 4 Radiation injury Oral administration |
| FundProject:国家杰出青年科学基金资助项目(39725014);国家自然科学基金资助项目(39780007);国家863资助项目(2001AA215311,2002AA223354);国家973资助项目(001CB5101) |
| Author Name | Affiliation | E-mail | | ZHAO Li-hua | State Key Laboratory of Experiment Hematology, Chinese Academy of Medical Sciences, TianJin 300020, China | tihzchan@public.tpt.tj.cn | | LIU Bin | State Key Laboratory of Experiment Hematology, Chinese Academy of Medical Sciences, TianJin 300020, China | | | YU Xiao-fei | State Key Laboratory of Experiment Hematology, Chinese Academy of Medical Sciences, TianJin 300020, China | | | 张磊 | State Key Laboratory of Experiment Hematology, Chinese Academy of Medical Sciences, TianJin 300020, China | | | 韩忠朝 | State Key Laboratory of Experiment Hematology, Chinese Academy of Medical Sciences, TianJin 300020, China | |
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| Abstract:: |
| Objective To investigate the in vivo radiation protection effect of PF4 by oral administration of attenuated salmonella as the carrier in mice. Methods The eukaryotic vector pIRES2-EGFP-carried PF4 gene was transferred into an aroA-autotrophic mutant of salmonella typhimurium (SL3261), which was administered orally to BALB/c mice at 1×108 PFu once every interval three days. At 12 hours after the third oral administration the mice were subjected to a total body irradiation (TBI) of 700 cGy by a 60Co source. The protective effect of SL3261/PF4 was determined by detection GFP(green fluorescence protein) expression in tissues, peripherial blood count, culture of bone marrow colony-forming cells and survival time of mice. Results (Expression of) GFP could be detected in the liver, spleen, intestine, kidney, peripheral blood and bone marrow. On days 7 and 14 after irradiation, Compared to controls, there were obvious differences in number of bone marrow mononuclear cells, CFU-GM (granulocyte-macrophage colony-forming unit) and HPP-CFC (high proliferating potential-colony-forming cells) of mice treated with SL3261/PF4 (P<0.05) as well as prolongation of the survival time. Conclusion These data demonstrate for the first time that PF4 protects mice from TBI injury and accelerates recovery of hematopoiesis by oral administration of attenuated salmonella carrying PF4 gene. |
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