| HUANG Shan_ying,SONG Liang_wen,ZHANG Yong.Effect of connective tissue growth factor (CTGF) expression on radiation pulmonary fibrosis in rats[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):213-216 |
| Effect of connective tissue growth factor (CTGF) expression on radiation pulmonary fibrosis in rats |
| Received:November 01, 2004 |
| DOI： |
| KeyWords:Radiation pulmonary fibrosis CTGF Fibroblasts Transformation Collagen |
| FundProject:国家自然科学基金资助项目(30170292);军事医学科学研究基金资助项目(2002) |
| Author Name | Affiliation | E-mail | | HUANG Shan_ying | Department of Pathology, Beijing Institute of Rdiation Medicine, Beijing 100850, China | Lsong1@yahoo.com | | SONG Liang_wen | Department of Pathology, Beijing Institute of Rdiation Medicine, Beijing 100850, China | | | ZHANG Yong | Department of Pathology, Beijing Institute of Rdiation Medicine, Beijing 100850, China | | | 孙丽 | Department of Pathology, Beijing Institute of Rdiation Medicine, Beijing 100850, China | | | 李杨 | Department of Pathology, Beijing Institute of Rdiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the effect of connective tissue growth factor(CTGF) on initiation of radiation pulmonary fibrosis (RPF) and the relation to α-smooth muscle actin (α-SMA). Methods The promotive effect of CTGF on proliferation of human lung fibroblasts (HLF) by 5 Gy of 60Coγ-rays was determined by MTT colorimetry.The expressions of CTGF and α-SMA in HLF were observed by Western blot.Changes of collagen Ⅰ and Ⅲ in rat lungs were determined by Sirius red staining and polarization microscopy.Expressions of CTGF and α-SMA in RPF were observed with immuneohisto-chemistry and analysis. Results Expressions of CTGF and α-SMA were increased.CTGF reached its peak at 24 h after irradiation,whereas α-SMA still kept at a highlevel 72 h after irradiation.A small amount of collagen was produced in rat lung one month after irradiation,in which type Ⅲ collagen was the primary component.However,a large amount of collagen was produced in rat lung 3-6 months after irradiation,in which type Ⅰ was dominant.CTGF began to expression 1 week after irradiation in proliferative fibroblasts of rat lung,and it was most evident 3 months after irradiation.α-SMA began to express in proliferative myofibroblasts 1 week after irradiation, and the high peek was reached at 3 months after irradiation. Conclusion Irradiation can induce expression of CTGF in pulmonary tissue and the later can promote the transformation of fibroblasts to myofibroblasts,strengthen the ability of synthesis and secretion of type Ⅰ and Ⅲ collagen. |
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