| YANG Wei,PIAO Chun-ji,LIU Lin_lin,et al.Expression of a recombinant dual-gene co-expressing plasmid pEgr-IFN γ-endostatin in Lewis lung cancer cells induced by radiation[J].Chinese Journal of Radiological Medicine and Protection,2005,25(3):210-212 |
| Expression of a recombinant dual-gene co-expressing plasmid pEgr-IFN γ-endostatin in Lewis lung cancer cells induced by radiation |
| Received:June 12, 2004 |
| DOI： |
| KeyWords:Dual-gene co-expression plasmid pEgr-IFNγ-endostatin Lewis lung cancer cells X-rays Expression induced by radiation |
| FundProject:国家自然科学基金资助项目(30170290) |
| Author Name | Affiliation | E-mail | | YANG Wei | MH Radiobiology Reserch Unit, School of Public Health, Jilin University, Changchun 130021, China | drxyli@yahoo.com.cn | | PIAO Chun-ji | MH Radiobiology Reserch Unit, School of Public Health, Jilin University, Changchun 130021, China | | | LIU Lin_lin | MH Radiobiology Reserch Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 田梅 | MH Radiobiology Reserch Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 潘艳 | MH Radiobiology Reserch Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 李修义 | MH Radiobiology Reserch Unit, School of Public Health, Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To construct a recombinant dual-gene co-expressing plasmid pEgr-IFN γ-endostatin and detect its radiation-induced expression in Lewis lung cancer cells. Methods The recombinant plasmid pEgr-IFN γ-endostation containing Egr-1 promoter,IFNγ and endostatin genes was constructed with gene recombination technique.The plasmid was transferred into Lewis lung cancer cells by liposme in vitro. The correlation of dose and effects and the time-course patterns of the expressions of IFNγ and endostatin genes induced by different doses of X-rays were detected by ELISA. Results Identification with enzymes proved that Egr-1 promoter,IFNγ and endostatin genes were inserted into the dual-gene co-expressing vector pIRESlneo correctly.After different doses of X-irradiation,the expressions of IFNγ and endostatin the supernatant of cultured Lewis lung cancer cells transfected by pEgr-IFNγ-endostatin were significantly higher than those in 0 Gy group.After 5 Gy X-irradiation,the expressions of IFNγ and endostatin were the highest,being 4.14 and 2.92 times as much as those in 0 Gy group respectively.The concentrations of IFNγ and endostatin in the supernatant increased after 2 Gy X-irradiation,being 3.75 and 3.02 times as much as those in 0 Gy group respectively 36 h after irradiation (P<0.001). Conclusion The dual-gene co-expressing plasmid pEgr-IFN γ-endostatin has been constructed successfully,and it has the property of enhancing the co-expression of IFNγ and endostatin genes induced by irradiation. |
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