| YUE Lu,SHEN Zan,CHEN Hong-hong.Research on apoptosis of MCF-7 cells induced by continuous irradiation by 125I seeds[J].Chinese Journal of Radiological Medicine and Protection,2005,25(2):130-132 |
| Research on apoptosis of MCF-7 cells induced by continuous irradiation by 125I seeds |
| Received:March 20, 2004 |
| DOI： |
| KeyWords:I seed Apoptosis The ratio of Bcl-2/Bax |
| FundProject:上海市科技发展基金资助项目(994119002) |
| Author Name | Affiliation | | YUE Lu | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | SHEN Zan | 上海市第一人民医院肿瘤科 | | CHEN Hong-hong | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | 罗伟华 | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | 程文英 | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China |
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| Abstract:: |
| Objective To detect the apoptotic rate and the changes of expressions of Bcl-2 and Bax in MCF-7 cells continuously irradiated with low dose rate γ-rays from 125I seeds. Methods MCF-7 cells were exposed to the radiation for 72 hours from model 6711 125I seeds with an apparent activity of 27.75 MBq. Then, RT-PCR and Western blot were used to detect the expressions of Bcl-2 and Bax in MCF-7 cells. FCM and Annexin V assay were performed to detect the apoptotic rate of MCF-7 cells. Results Higher expression of Bcl-2 and lower expression of Bax in irradiated MCF-7 cells compared to those in the control cells were detected. FCM and Annexin V assay showed a significant increase of apoptotic rate in MCF-7 cells after continuous irradiation by 125I seeds. Conclusion Continuous irradiation with 125I increases the apoptotic rate of MCF-7 cells, and this increase may be induced by decreased ratio of Bcl-2/Bax. |
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