| YIN Li-jie,XU Xiao-ying,DING Tian-gui,et al.Relationship between irradiation dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells in vitro[J].Chinese Journal of Radiological Medicine and Protection,2005,25(2):127-129 |
| Relationship between irradiation dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells in vitro |
| Received:August 15, 2004 |
| DOI： |
| KeyWords:Pancreatic carcinoma cell Apoptosis bcl-2 bax |
| FundProject: |
| Author Name | Affiliation | | YIN Li-jie | Department of Radiotherapy, Dalian Central Hospital, Dalian 116033, China | | XU Xiao-ying | 116033, 大连医科大学附属二院肿瘤科 | | DING Tian-gui | Department of Radiotherapy, Dalian Central Hospital, Dalian 116033, China | | 朱晓钰 | 116033, 大连大学医学院附属大连中心医院实验室 | | 纪军 | 116033, 大连大学医学院附属大连中心医院实验室 | | 邹丽娟 | 116033, 大连医科大学附属二院肿瘤科 |
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| Abstract:: |
| Objective To explore the relationship between irradition dose and expressions of bcl-2,bax genes in apoptosis process of pancreatic carcinoma cells. Methods Apoptosis was detected with PI and AnnexinⅤ/PI methods and bcl-2,bax proteins in Panc-1 cells after gamma-irradiation were determined with flow cytometry. Results The results showed that the apoptotic rate increased along with the increase of dose and time within a certain range (≤15 Gy,≤24 h). The expression of bcl-2 was significantly lower in the irradiated cells than that in the control cells (P<0.05),but that of bax was higber in the former than that in the latter (P<0.05). Conclusion Gamma-radiotherapy can induce apoptosis in pancreatic carcinoma cells through down-regulation of the expression of bcl-2 and up-regulation of the the expression of bax. |
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