| WU Ning,SUN Han-ying,LIU Wen-li.Effects of ligustrazine on expression of endostatin in syngenic bone marrow-transplated mice[J].Chinese Journal of Radiological Medicine and Protection,2005,25(2):121-123 |
| Effects of ligustrazine on expression of endostatin in syngenic bone marrow-transplated mice |
| Received:June 11, 2004 |
| DOI： |
| KeyWords:Ligustrazine Bone marrow transplatation Hematopoietic reconstitution Endostatin |
| FundProject:国家自然科学基金资助项目(39870926) |
| Author Name | Affiliation | | WU Ning | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | SUN Han-ying | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | LIU Wen-li | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 何莉 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 孟凡凯 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 李春蕊 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 刘丹 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 付丽 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 罗琳 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | 徐惠珍 | Dapartment of Hematology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract:: |
| Objective To explore the effect of ligustrazine on expression of endostatin in bone marrow stromal cells of mice after syngenic bone marrow transplatation(BMT). Methods The mice were randomly divided into 3 group:normal group (without any treatment),BMT control group and ligustrazine-treated groups.A BMT mouse model was established.The mice in the BMT control group and the ligustrazine-treated group were orally given 0.2 ml saline and 2 mg ligustrazine,respectively,twice a day.On days 7,14,21,28 after BMT,mice were sacrificed.Bone marrow nucleated cells (BMNCs) were counted and the expression of endostatin was assayed by immunohistochemistry,Western blot and RT-PCR analysis respectively. Results On the days 7,14,21,28 d after BMT,expression of endostatin in bon marrow stromal cells was significantly lower than that in the normal group(P<0.05 or P<0.01).In the ligustrazine-treated group,the number of BMNCs on the days 7,14,21,28 after BMT was higher than that in the BMT control group (P<0.05 or P<0.01).However the expression of endostatin in the former was lower than that in the BMT control group (P<0.05). Conclusion Ligustrazine can inhibit endostatin expression in bone marrow stromal cells after syngenic BMT in mice,which may be related to the ligustrazine′s accelerating hematopoietic reconstitution in mice after BMT. |
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