| HE Gui-jin,ZHANG Hong,GAO Qin-yi.The role of γ-ray-induced fibroblast apoptosis in inhibiting biliary duct hypertrophic scar formation in dogs[J].Chinese Journal of Radiological Medicine and Protection,2005,25(2):118-120 |
| The role of γ-ray-induced fibroblast apoptosis in inhibiting biliary duct hypertrophic scar formation in dogs |
| Received:March 15, 2004 |
| DOI： |
| KeyWords:Apoptosis γ-radiation 103Pd radioactive stent |
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| Author Name | Affiliation | | HE Gui-jin | Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China | | ZHANG Hong | Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China | | GAO Qin-yi | 中国医科大学第一临床学院同位素室 | | 许书河 | 中国原子能研究院同位素研究室 | | 高红 | 110004 沈阳, 中国医科大学第二临床学院儿外实验室 | | 姜维国 | 110004 沈阳, 中国医科大学第二临床学院病理科 | | 蒋涛 | 110004 沈阳, 中国医科大学第二临床学院儿外实验室 | | 戴显伟 | Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China | | 马凯 | Department of General Surgery, Second Clinical College, China Medical University, Shenyang 110004, China |
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| Abstract:: |
| Objective To investigate the role of γ-ray-induced fibroblast apoptosis in the inhibition of biliary duct hypertrophic scar formation in dogs. Methods γ-radiation-induced apoptotic fibroblast cells were analysed by using transmission electron microscopy and DNA from frozen biliary duct tissue was extracted with phenol chloroform.DNA ladder profile after extraction of RNA was observed,and apoptosis cells in paraffineem-bedded biliary duct tissue sections were examined using immuno-histochemical method.Dog biliary duct cross-sections were stained with hematoxylin-erosin,Masson’s trichrome,and Verhoeff-van Giesen stains.Muscle formation area,lumen circumference,and stenosis degree were determined by a computer-assisted image analysis system. Results 103Pd radioactive stent significantly inhibited fibroblast proliferation.The features of fibroblast apoptosis (e.g.apoptic bodies,DNA ladder band) could be seen in the 103Pd radioactive stent group.The fibroblast apoptotic rate was significantly increased in the 103Pd radioactive stent group than in the control group(P<0.01).Comparing with the control group,the 103Pd radioactive stent significantly reduced biliary muscular formation. Conclusion 103Pd radioactive stent could have the effect of inhibiting the proliferation of scar-forming fibroblast,and thus could be used for treatment and (or) prevention of hypertrophic scar formation in biliary duct. |
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