| ZONG Zhao-wen,CHENG Tian-min,RAN Xin-ze,et al.Experimental study on chemotactic effect of wound extract from rat with combined injury on dermal multipotent stem cells[J].Chinese Journal of Radiological Medicine and Protection,2005,25(2):111-113 |
| Experimental study on chemotactic effect of wound extract from rat with combined injury on dermal multipotent stem cells |
| Received:September 17, 2004 |
| DOI： |
| KeyWords:Combined injury Dermal multipotent stem cell Wound extract Chemotaxis |
| FundProject:国家重点基础研究发展规划“973”专项资助项目(G1999054205);国家自然科学基金资助项目(30370562) |
| Author Name | Affiliation | | ZONG Zhao-wen | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | CHENG Tian-min | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | RAN Xin-ze | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 粟永萍 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 徐辉 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 史春梦 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 屈纪富 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To observe the chemotactic effect of wound extract from rat with combined injury on dermal multipotent stem cells (dMSCs),and to investigate the effect of transplanted dMSCs on wound healing. Methods Using Transwell microchemotaxis chamber system,we observed the effect of wound extract on the migration of dMSCs.Rat dMSCs labeled with 3H-TdR were transplanted into rats and their distribution in various tissues was measured by liquid scintillation.In addition,the degree of wound healing was observed by measuring the remaining wound area. Results The wound extract significantly promoted migration of dMSCs across the membrane of the microchemotaxis chamber when compared with normal tissue extract and saline (P<0.01).From 1 week after injury,the amount of dMSCs in rat wounds of combined injury was larger than that of the normal control rats (P<0.01).As far as per gram tissue was concerned,the amount of dMSCs in wound was greater than that in lung and liver in rats with combined-injury 3 and 4 weeks after the injury.The remaining wound area in dMSCs-transplanted rats was smaller than that of non-transplanted rats from day 15 after injury (P<0.05). Conclusion Our results suggest that wound extract form rat with combine injury can enhance the migration of dMSCs,and thus promotes transplanted dMSCs to migrateto wound area and promote wound healing significantly. |
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