| YUAN Jian,SUN Ning.Study on radiosensitivity heterogeneity of the NPC cell line CNE-2Z and the related mechanism[J].Chinese Journal of Radiological Medicine and Protection,2005,25(1):52-55 |
| Study on radiosensitivity heterogeneity of the NPC cell line CNE-2Z and the related mechanism |
| Received:June 07, 2004 |
| DOI： |
| KeyWords:Nasopharyngeal carcinoma Heterogeneity Radiosensitivity Apoptosis Oncogene DNA |
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| Abstract:: |
| Objective To further confirm that the human NPC cell line CNE-2Z indeed has radiosensitivity heterogeneity and to discuss its related mechanism. Methods To observe the difference between sublines H5 and S1 of the cell line CNE-2Z in the radiosensitivity of their transplanted tumors in nude mice, the difference between H5 and S1 in their apoptosis-inducing capacity and that in apoptosis-related protein expression were detected by FCM, fluorescence microscope and Western-blot. The difference in their DNA synthesis inhibition rate and radiosensitivity were expressed by tritium incorporation method. The relationship between the expression of related oncogenes and radiosensitivity was observed by RT-PCR. Results Radiosensitivity heterogeneity did exist in NPC cell line CNE-2Z, and the apoptosis rate of both types of cells, H5 and S1, increased with extension of after-irradiation time, but H5 had a higher apoptosis rate than S1 (P<0.05). There was no difference in DNA synthesis rate between the two cell types; after irradiation, however, the DNA synthesis rate of both cell types was inhibited compared with that before irradiation(P<0.05), especially that of H5 being inhibited to a greater degree (P<0.05). The expression levels of genes fas and p53 of H5 and S2 cells did not have statistical significance (P>0.05). Conclusion The present experiment has once again proved that NPC cell line CNE-2Z indeed has radiosensitivity heterogeneity and also proved that the heterogeneity is related to the capacity of cells in inducing apoptosis after irradiation and is positively correlated with the inhibition rate of DNA synthesis but is unrelated to the expression of oncogenes fas and p53. |
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