| LIU Tao,SONG Liang-wen,DONG Jun-xing,et al.Preventive and curative effects of dicaffeoylquinic acid on early pulmonary fibrosis in mice[J].Chinese Journal of Radiological Medicine and Protection,2005,25(1):36-39 |
| Preventive and curative effects of dicaffeoylquinic acid on early pulmonary fibrosis in mice |
| Received:February 28, 2004 |
| DOI： |
| KeyWords:Dicaffeoylquinic acid α-smooth muscle actin Matrilysin Pulmonary fibrosis Prevention and treatment |
| FundProject:军事医学科学开发研究基金资助项目(2002) |
| Author Name | Affiliation | E-mail | | LIU Tao | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | Lsong1.@yahoo.com | | SONG Liang-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | DONG Jun-xing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 黄山英 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 李杨 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the effect of dicaffeoylquinic acid(IBE5) on prevention and treatment of pulmonary fibrosis induced by bleomycin(BLM) in mice and its mechanism. Methods Hydroxyproline content determination,imaging analysis,collagen Ⅰand Ⅲ assay,α-smooth muscle actin(α-SMA) and matrix metalloproteinase 7(MMP-7) immunohistochemistry were performed. Results ①Hydroxyproline content decreased in fibrotic lung tissue after administration of IBE5(P<0.05).②The number of pulmonary alveoli reduced,alveolus interstitium was thickened and collagen deposition and fibrosis were formed in lung tissue of BLM group. The break of pulmonary alveoli and extension of pulmonary fibrosis were decreased by use of IBE5 (P<0.05).③A lot of collagen Ⅰand Ⅲ were synthesized in lung interstitium in BLM group and their quantity was reduced in IBE5 group (P<0.05).④In BLM group,α-SMA expression increased and located in myofibroblasts in fibrotic area,and MMP7 immunohistochemical signal was located in myofibroblasts also. They were decreased in IBE5 group(P<0.05). Conclusion IBE5 plays a preventive and curative role in pulmonary fibrosis by inhibition of transformation of fibroblasts towards myofibroblasts and MMP7 expression. |
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