| FU Zhichao,CAI Jianming,HAN Ling,et al.Quantification of extracellular signalregulated kinase 2 gene in the tumor tissue of leukemia of mice induced by 60Co γ-rays using fluorogenic quantitative RTPCR method[J].Chinese Journal of Radiological Medicine and Protection,2004,24(6):550-553 |
| Quantification of extracellular signalregulated kinase 2 gene in the tumor tissue of leukemia of mice induced by 60Co γ-rays using fluorogenic quantitative RTPCR method |
| Received:November 11, 2003 |
| DOI： |
| KeyWords:Radiation carcinogenesis Signal transduction Extracellular signalregulated kinase Leukaemia Reverse transcriptpolymerase chain reaction |
| FundProject: |
|
| Hits: 5778 |
| Download times: 2293 |
| Abstract:: |
| Objective to establish a fluorogenic quantitative RTPCR(FQPCR) method for detecting the expression of ERK2 gene in tumor tissues and investigate the relationship between ERK2 mRNA expression and leukemia induced by γ-ray irradiation in mice. Methods After establishment of a leukemia model induced by γ-ray irradiation in mice,the animals were divided into three groups:the canceration group,the uncanceration group and the unirradiated group according to the pathological examination.Fluorogenic quantitative RTPCR(FQPCR) was set up to examine the expression of ERK2 in the tumor tissue of the leukemia induced by γ-ray irradiation in mice. Results The level of the ERK2 gene in the thymus and bone marrow cells of leukemic mice was significantly higher than in that of the control groups(P.<0.05). Conclusion The result of ERK2 gene expression detedcted with FQPCR is accurate and dependable.ERK2 may be involved in the canceration of irradiated mice. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
