| FENG Jiangbin,LU Xue,CHEN De-qing.Detection the human mitochondrial DNA 4977 bp deletion induced by 60Co γ-rays in vitro by nest-PCR[J].Chinese Journal of Radiological Medicine and Protection,2004,24(6):533-536 |
| Detection the human mitochondrial DNA 4977 bp deletion induced by 60Co γ-rays in vitro by nest-PCR |
| Received:May 09, 2004 |
| DOI： |
| KeyWords:Mitochondrial DNA 4977 bp deletion 60Co γ-rays Nest-PCR |
| FundProject:人事部留学回国人员择优资助项目(2002年 优秀类) |
| Author Name | Affiliation | E-mail | | FENG Jiangbin | National Institute for Radiological Protection, China CDC, Beijing 100088, China | piegjiu@yahhoo.cn | | LU Xue | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | CHEN De-qing | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 陈晓穗 | 海军总医院 | | | 刘青杰 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | |
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| Abstract:: |
| Objective To establish a method for detecting the mitochondrial DNA 4977 bp deletion (mtDNA 4977) induced by different doses of ionizing radiation. Methods A nest-PCR method was established with 3 primer pairs for detecting the human peripheral mtDNA 4977.The final PCR products were sequenced after purified and the sequence was BLASTed with the standard genome information of human mitochondrion. The mtDNA 4977 level induced by 05 Gy 60Co γ-rays of 5 healthy individuals was analyzed with the established nest-PCR. Results The mtDNA 4977 could be detected by the established nest-PCR method. The mtDNA 4977 was observed on samples after exposed to 15 Gy 60Co γ-rays,but it was not observed before (0 Gy) exposure. Conclusion The nest-PCR method established in this study could be used to detect the mtDNA 4977 induced by ionizing radiation. |
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