| FAN Cai,ZHANG Jianjun,LI Xiu-li,et al.Effect of ionizing radiation on MT-1 mRNA expression of mouse tissues[J].Chinese Journal of Radiological Medicine and Protection,2004,24(6):525-526 |
| Effect of ionizing radiation on MT-1 mRNA expression of mouse tissues |
| Received:April 26, 2004 |
| DOI： |
| KeyWords:Metallothionein Ionizing radiation mRNA |
| FundProject:国家自然科学基金资助项目(39170275) |
| Author Name | Affiliation | | FAN Cai | Bethune International Peace Hospital, Shijiazhuang 050082, China | | ZHANG Jianjun | Bethune International Peace Hospital, Shijiazhuang 050082, China | | LI Xiu-li | Bethune International Peace Hospital, Shijiazhuang 050082, China | | 徐莉 | Bethune International Peace Hospital, Shijiazhuang 050082, China | | 陈卫辉 | Bethune International Peace Hospital, Shijiazhuang 050082, China | | 鞠桂芝 | 吉林大学放射生物学教研室 |
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| Abstract:: |
| Objective To investigate the effect of whole body X-irradiation (WBI) on metallothionein-1 (MT-1) gene transcription level in mouse tissues and its dose-effect relationship. Methods MT-1 mRNA levels in mouse liver,brain,thymus and spleen were measured using Northern blot assay. Results Time-course experiment showed that MT-1 mRNA levels in mouse liver and thymus gradually increased after WBI,reached a peak at 4 h after WBI and recovered normal level at 24 h. MT-1 mRNA levels in mouse liver and thymus increased with increase of irradiation dose at 4 h after 0.5～6 Gy X-ray WBI. But there was no obvious change of MT-1 mRNA levels in mouse brain and spleen. Conclusion MT-1 mRNA levels in mouse liver and thymus significantly increase after WBI and showed a dose-dependent trend. But there is no obvious change in MT-1 mRNA levels in mouse brain and spleen. These Results indicate that increase of MT-1 mRNA in mouse tissues after WBI is tissue-specific,and MT protein synthesis is regulated through MT-1 gene transcriptional elevation. |
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