| HUANG Yue-cheng,DONG Zhi-tao,CHU Wan-li.Expression of p53、MDM2 in a mice hydradecarcinoma model induced by γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(6):517-519 |
| Expression of p53、MDM2 in a mice hydradecarcinoma model induced by γ-ray irradiation |
| Received:September 10, 2003 |
| DOI： |
| KeyWords:p53 MDM2 Hydradecarcinoma Irradiation |
| FundProject:国家自然科学基金资助项目(30170282) |
| Author Name | Affiliation | | HUANG Yue-cheng | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | DONG Zhi-tao | 第二军医大学学员旅1队 | | CHU Wan-li | 第二军医大学学员旅1队 | | 蔡建明 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | 韩玲 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | 高福 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China | | 孙顶 | Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China |
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| Abstract:: |
| Objective To investigate the role of the p53,MDM2 in carcinogenesis of mice hydradecarcinoma induced by γ-rays. Methods A radiation-induced mice hydradecarcinoma model was established by γ-ray irradiation. Expression of MDM2 protein in hydradecarcinoma tissue,paracancerous tissue and normal control tissue was detected with Western blot. Immunoprecipitation (IP) was conducted to examine the phosphorylation level of MDM2 protein. PCR-SSCP was performed to detect p53 gene mutation. Results Compared with the normal control tissue,the MDM2 protein expression and its phosphorylation level were significantly higher in hydradecarcinoma tissue. SSCP showed there were p53 gene mutations in hydradecarcinoma samples. Conclusion p53/MDM2 pathway may be involved in the development and progression of hydradecarcinoma induced by γ-ray irradiation. The over-expression of MDM2 and hyperphosphorylation may be responsible for malignant transformation induced by irradiation by a possible mechanism of p53 inactivation. The gene mutation of p53 further supported the hypothesis that p53/MDM2 pathway played a central role in carcinogenesis of γ-ray induced hydradecarcinoma. |
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