| YANG Yepeng,ZHU Yingbao,CHEN Guanying.Comparative study on 4 quantitative detection methods of apoptosis induced by radiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(6):497-499 |
| Comparative study on 4 quantitative detection methods of apoptosis induced by radiation |
| Received:July 15, 2004 |
| DOI： |
| KeyWords:rays Tumor cells Apoptosis Necrosis Comparison between detecting methods |
| FundProject: |
| Author Name | Affiliation | | YANG Yepeng | Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China | | ZHU Yingbao | 北京大学第三医院中心实验室 | | CHEN Guanying | Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China | | 周梅 | Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China | | 沈嵚剑 | Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China | | 沈磊 | Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing 100083, China |
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| Abstract:: |
| Objective To reveal the capability of 4 apoptosisdetecting Methods to discriminate between apoptosis and necrosis and show their respective advantages and shortcomings through comparison of detected Results and analysis of detection mechanism. Methods Four Methods,PI stainingflow cytometric detection (P-F method),TUNEL labelingflow cytometric detection (T-F method),annexin VFITC/PI vital stainingflow cytometric detection (A-F method) and Hoechst/PI vital stainingfluorescence microscopic observation (H-O method),were used to determine apoptosis and necrosis in human breast cancer MCF7 cell line induced by γ-rays.Hydroxycamptothecine and sodium azide were used to induce positive controls of apoptosis and necrosis respectively. Results All 4 Methods showed good timedependent and dosedependent respondences to apoptosis induced by γ-rays and hydroxycamptothecine.Apoptotic cell ratios and curve slopes obtained from PF method were minimum and,on the contrary,those from TF method were maximum among these 4 Methods.With AF method and HO method,two sets of data,apoptosis and necrosis,could be gained respectively and the data gained from these two Methods were close to equal.A-F method and HO method could distinguish necrosis induced by sodium azide from apotosis while P-F method and T-F method presented false increase of apoptosis. Conclusions P-F method and T-F method can not discriminate between apoptosis and necrosis.P-F method is less sensitive but more simple,convenient and economical than T-F method.AF method and HO method can distinguish necrosis from apoptosis.AF method is more costly but more quick and reliable than H-O method.H-O method is economical,practical and morphological changes of cells and nucleus can be observed simultaneously with it. |
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