| ZHAO Jian,HAN Yun,ZHU Yingbao.Heterochromatic protein HP1α is involved in repair of radiation damage[J].Chinese Journal of Radiological Medicine and Protection,2004,24(6):493-495 |
| Heterochromatic protein HP1α is involved in repair of radiation damage |
| Received:July 15, 2004 |
| DOI： |
| KeyWords:Radiation damage repair Heterochromatin HP1α HR24L |
| FundProject:国家自然科学基金资助项目(30070232) |
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| Abstract:: |
| Objective To investigate the role of HP1α in DNA repair induced by ionizing radiation. Methods Inhibition of HP1α and HR24L by antisense RNA,respectively and combinatively was used to observe the sensitivity of cells to ionizing radiation,and coimmuoprecipitation was performed to find HP1αcontaining HR24L complex. Results Inhibition of HP1α expression had no obvious effect on radiation sensitivity of cells.Significantly,double inhibition of both HP1α and HR24L led to increased sensitivity of cells to ionizing radiation than sigle inbihition of HR24L.In addition,HP1α was found to coprecipitated with HR24L after DNA damage induced by ionizing radiation with 20 Gy γ-rays. Conclusion HP1α participates in the formation of HR24L complex that is involved in DNA damage repair,thus contributing to the radiation sensiivity of cells. |
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