| YUAN Chang-qing,DING Zhen-hua,DU Hua,et al.UVA-induced apoptosis via ceramide and JNK signaling pathway[J].Chinese Journal of Radiological Medicine and Protection,2004,24(5):455-457 |
| UVA-induced apoptosis via ceramide and JNK signaling pathway |
| Received:October 20, 2003 |
| DOI： |
| KeyWords:Ultraviolet radiation Apoptosis Ceramide JNK signaling pathway |
| FundProject:国家自然科学基金资助项目(30200226;39970189) |
| Author Name | Affiliation | E-mail | | YUAN Chang-qing | Department of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China | yuanchq@hotmail.com | | DING Zhen-hua | Department of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China | | | DU Hua | Department of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China | | | 凌朝辉 | Department of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China | | | 马树东 | Department of Radiation Medicine, Institute of Tropical Medicine, The First Military Medical University, Guangzhou 510515, China | |
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| Abstract:: |
| Objective To investigate the role of ceramide and JNK signaling pathway in UVA-induced apoptosis. Methods Cell death rate and apoptosis were examined by MTT assay and DNA ladder analysis respectively.Activation of JNK signaling pathway was analyzed by Western blot. Results UVA-induced apoptosis was markedly observed in lymphocyte JY,but not in MS 1418 cells from Niemann-Pick type D patients with an inherited deficiency of acid sphingomyelinase,resulting in inhibition of ceramide production.Once exogenous ceramide was administered,apoptosis occurred in both JY and MS 1418 cells,indicating UVA induced apoptosis via ceramide.UVA radiation activated JNK signaling pathway in JY but not in MS 1418, and exogenous ceramide stimulated JNK signaling pathway in both cells.The results indicated that ceramide was on the top of JNK signaling pathway. Conclusion UVA-induced apoptosis depends on ceramide and JNK signaling pathway. |
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