| WU Cong-mei,HUANG Tian-hua,XIE Qing-dong.Construction of pETNF-P16 plasmid and its expression properties in esophageal carcinoma cells induced by ionizing irradiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(5):406-408 |
| Construction of pETNF-P16 plasmid and its expression properties in esophageal carcinoma cells induced by ionizing irradiation |
| Received:October 04, 2003 |
| DOI： |
| KeyWords:Egr-1 promoter X-ray TNFα P16 Esophageal carcinoma |
| FundProject:国家自然科学基金资助项目(30210103904);广东省科技计划资助项目(2003C30304) |
| Author Name | Affiliation | | WU Cong-mei | Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515031, China | | HUANG Tian-hua | Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515031, China | | XIE Qing-dong | Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515031, China | | 李德锐 | 515031, 汕头大学医学院附属肿瘤医院 | | 吴德生 | Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515031, China | | 徐小虎 | 515031, 汕头大学医学院法医教研室 515031, |
|
| Hits: 3038 |
| Download times: 1623 |
| Abstract:: |
| Objective The recombined plasmid pETNF-P16 was constructed to investigate its expression properties in esophageal squamous cell carcinoma cell line EC9706 induced by X-ray irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. Methods The recombined plasmid pETNF-P16 was constructed and transfected into EC9706 cells with lipofectamine.ELISA,Western blot,and immunocytochemistry were performed to determine the expression properties of pETNF-P16 in EC9706 after transfection induced by X-ray irradiation. Results The eukaryotic expression vector pETNF-P16 was successfully constructed and transfected into EC9706 cells.The expressions of TNFα were significantly increased in the transfected cells after different dose of X-ray irradiation than that of 0 Gy group(P<0.05～0.01) and the expressions of TNFα and P16 were significantly higher during 2～48 h after 2 Gy X-ray irradiation than that of control group (P<0.05～0.01).No P16 expression in normal EC9706 cells and strong expression in the transfected group and irradiation induction group were detected. Conclusions X-ray irradiation induction could significantly enhance the TNFα and P16 expression property in the EC9706 cells transfected with pETNF-P16 plasmid.These results may establish important experimental basis for gene-radiotherapy of esophageal carcinoma. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
