| DONG Li-hua,LIU Guang-wei,PIAO Chun-nan.Effect of signal factors on lymphocyte apoptosis in mouse thymus induced by ionizing radiation in vitro[J].Chinese Journal of Radiological Medicine and Protection,2004,24(5):399-402 |
| Effect of signal factors on lymphocyte apoptosis in mouse thymus induced by ionizing radiation in vitro |
| Received:February 04, 2004 |
| DOI： |
| KeyWords:Signal factors Ionizing radiation Lymphocytes Apoptosis |
| FundProject:国家自然科学基金资助项目(39170275) |
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| Abstract:: |
| Objective To observe the effects of corticosterone (CS),cAMP,cGMP,Ca2+ and protein kinas C(PKC) signal factors on lymphocyte apoptosis in mouse thymus induced by ionizing radiation of 4 Gy X-rays in vitro. Method The DNA lytic rate of lymphocytes in thymus was measured with fluorospectrophotometry. Results The DNA lytic rates of lymphocytes in thymus 4-8 h after irradiation with 2-8 Gy were all significantly higher than those of the control.As compared with the control,the DNA lytic rates of lymphocytes all increased significantly in 0.01 μmol/L CS(P<0.01),50 ng/ml cAMP (P<0.01),0.05-0.4 μg/ml ionomycin (Iono,(P<0.05) or P<0.01) and 0.05-0.4 ng/ml phorbol myristate acetate (PMA,P<0.05 or P<0.01) groups,respectively,while the rate in 50 ng/ml cGMP did not increase significantly.The DNA lytic rates of lymphocytes were significantly higher in 0.01 μmol/L CS(P<0.01),50 ng/ml cAMP (P<0.01),0.2 and 0.4 μg/ml Iono (P<0.05) and 0.2 and 0.4 ng/ml PMA (P<0.05) plus 4 Gy-irradiation groups respectively than that in single 4 Gy-irradiation group,while that in 50 ng/ml cGMP plus 4 Gy-irradiation group was not.As both Iono and PMA were combined to act to the lymphocytes,the DNA lytic rate of only in 0.4 μg/ml Iono and 0.4 ng/ml PMA group was significantly higher than that in the control (P<0.01);the rate in 0.4 μg/ml Iono and 0.4 ng/ml PMA plus 4 Gy-irradiation group was significantly higher than that in the single 4 Gy-irradiation group (P<0.05),but was not significantly higher than that in the 0.4 μg/ml Iono plus 4 Gy-irradiation group or 0.4 ng/ml PMA plus 4 Gy-irradiation group. Conclusion There are promoting effects of CS,cAMP,Ca2+ and PKC signal factors in certain dose ranges on lymphocyte apoptosis in mouse thymus induced by ionizing radiation with larger dose of X-rays. |
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