| ZHANG Feng,MENG Xiang-bing,Song Yi,et al.Involvement of cAMP-PKA in regulation of p27Kip1 expression induced by ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(5):393-395 |
| Involvement of cAMP-PKA in regulation of p27Kip1 expression induced by ionizing radiation |
| Received:January 05, 2004 |
| DOI： |
| KeyWords:Ionizing radiation p27Kip1 PKA Protein phosphorylation |
| FundProject:国家自然科学基金资助项目(30070239;30170291) |
| Author Name | Affiliation | E-mail | | ZHANG Feng | Beijing Institute of Radiation Medicine, Beijing 100850, China | sunzx@nic.bmi.ac.cn | | MENG Xiang-bing | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | Song Yi | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 梅柱中 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 董燕 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 刘斌 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 孙志贤 | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To study the mechanism of p27Kip1 down-regulation induced by ionizing radiation(IR). Methods The cDNA of p27Kip1 was cloned into prokaryotic expression vector pGEX4T-2.The recombinant protein GST-p27Kip1 was induced by isopropyl-β-D-thiroglactoside (IPTG) and purified by GST-Sepharose 4B affinity chromatography. Results GST-p27Kip1 was phosphorylated by PKAc,and this in vitro phosphorylation could be inhibited by PKA specific inhibitor H89.Phosphorylation level of GST-p27Kip1 decreased after IR,when treated with immunoprecipited endogenous PKA. Conclusion The results suggest that cAMP-PKA plays a critical role in down-regulation of p27Kip1 during IR. |
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