| YAO Li,LIANG Xiao-li,WANG De-wen,et al.Quantitative study of the effects of high power EMP on hepatocellular DNA content and ploidy in mice[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):386-388 |
| Quantitative study of the effects of high power EMP on hepatocellular DNA content and ploidy in mice |
| Received:October 16, 2003 |
| DOI： |
| KeyWords:Electromagnetic irradiation Electromagnetic pulse (EMP) Liver DNA content DNA diploid cell type Image cytophotometry(ICM) |
| FundProject:全军“十五”军队科研基金资助项目(01MB143,01L023) |
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| Abstract:: |
| Objective To observe the changes of high power electromagnetic pulse(EMP) on DNA content and DNA ploidy of mice hepatocytes and explore the biological effects of electromagnetic radiation on liver. Methods Kunming mice were exposed to whole body irradiation by a HEMP generator at electric field intensity of 8×10 3 V/m, 2×10 4 V/m, 6×10 4 V/m, respectively. The main relative technological parameters were: the pulse increase time was 20 nsec, pulse width 30 μs; and irradiation frequency 5 times in 2 min. The changes of hepatocellular nuclear DNA content in mice were analyzed for 365 days including 10 time phases (n =6)by using Feulgen staining in liver tissue sections. DNA content quantitation and DNA ploidy were measured by an IBAS microscopical digital image analysis system from German. Results Within 90 days after HEMP irradiation, the hepatocellular DNA content of mice were similar to that of the controls and the 2 C were dominant. On day 180 after irradiation, the higher DNA content (P <0.05) and more 4 C, 6 C than controls in 8×10 3 V/m group were found. On days 270 and 365, in all irradiation groups, the highest DNA content (P <0.01) and most 4 C were presented than the other time phases. Conclusion The results suggest that the DNA content and ploidy of hepatocyte in mice can be altered by HEMP and thus may be the long-term effects. It is proposed that the hepatocellular nucleic acid may be a key target for the biological effects of electromagnetic fields. The data provide basic information for studying the effects and mechanisms of EMP. Moreover, the investigation demonstrated that long-term observation must be emphasized in order to keep the integrity and reliability of results for gainning further insight into prevention and therapy for electromagnetic irradiation injury. |
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