| ZHAO Yong-cheng,TIAN Su-qing,WANG Ji-xian,et al.Study on the applications of FISH micronucleus technique in radiation biological dosimetry[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):333-335 |
| Study on the applications of FISH micronucleus technique in radiation biological dosimetry |
| Received:April 08, 2003 |
| DOI： |
| KeyWords:Ionization radiation Fluorescence in situ hybridization MN assay X-ray workers Biological dosimeter |
| FundProject:日本体质研究会基金资助项目;国家自然科学基金资助项目(30070644) |
| Author Name | Affiliation | | ZHAO Yong-cheng | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China | | TIAN Su-qing | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China | | WANG Ji-xian | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China | | 范亚光 | Institute of Radiation Medicine, Chinese Academy of Medicine Sciences, Tianjin 300192, China |
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| Abstract:: |
| Objective To investigate the feasibility of FISH micronucleus technique as a bio-dosimeter and evaluation the radiation effect of occupational population who exposed to radiation. Methods human peripheral blood samples were irradiated in vitro with 137 Cs γ rays with the doses ranging from 0.1 to 2.5 Gy, then CBMN was analyzed using FISH with Pancentromere DNA probe, and dose-response equation was estimated. And by detecting of the centromere-negative micronuclei rate of occupational exposure of the medical X-ray diagnostic workers in our country, the biological individual dose was estimated. Results Irradiatied in vitro, the micronucleus frequencies increased with the increase of radiation dose, and centromere-negative micronuclei accounted for the main increase of micronucleus and centromere-positive micronuclei only slightly increased. the dose-response relationships for CBMN assay and FISH assay were: YCBMN = 0.0048 + 0.0362D + 0.0109D2; R 2= 0.99; YFISH = 0.0007+ 0.0353D +0.0070D 2, R2= 0.99, respectively. Conclusions FISH micronucleus assay decreased the individual variation of spontaneous micronucleus, enhanced the radiation specificity of MN assay and was better than CBMN. The result shows that centromere-negative micronuclei rate can be used to estimate to biological dose. |
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