| LIU Jian- xiang,XUE Li- xiang,SU Xu.Ionizing radiation regulated expression of Lipofectamin-mediated p16 gene in HeLa cells[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):327-330 |
| Ionizing radiation regulated expression of Lipofectamin-mediated p16 gene in HeLa cells |
| Received:January 18, 2004 |
| DOI： |
| KeyWords:Egr-1 gene p16 gene p16 mRNA Cell cycle Cell growth curve |
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| Abstract:: |
| Objective To investigate the expression of Egr-p16 and its role in tumor cell inhibition in HeLa cells. Methods HeLa cells were transfected with p16 by recombined plasmids with Lipofectamin induced by γ-ray irradiation with different doses. RT-PCR was used to detect p16 mRNA level in the dose-effect and time-course aspects. Cell proliferation was detected by cell count and cell cycle changes were detected by FCM. Results The results showed that p16 mRNA level was higher than the control after 0.5-8.0 Gy irradiation. There were peaks after 2-4 Gy irradiation, and p16 mRNA level increased at 2-4 h after 2 Gy irradiation. There was a significant increase at 4 h. After the stably transfected HeLa cells were irradiated, the transfer of Egr-p16 combined with γ-ray irradiation could inhibit the growth of HeLa cells. The percentage of G0/G1 showed a dose-dependant decrease, but the percentage of S showed a dose-dependant increase. The percentage of G2/M showed a markedly arrest after 2 Gy irradiation, and gradually reduced after 5 and 10 Gy irradiation, but it was still higher than the control group. Conclusion γ-rays can induce the recombined plasmid to express at mRNA level in HeLa cells and there was a significant change in cell proliferation. |
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