| ZHONG Zhao-dong,ZOU Ping,YOU Yong,et al.Repairing of TBI-induced damage and remodeling of bone marrow-microvasculature by adenovirus mediated hVEGF165 gene therapy in post-BMT mice[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):316-318 |
| Repairing of TBI-induced damage and remodeling of bone marrow-microvasculature by adenovirus mediated hVEGF165 gene therapy in post-BMT mice |
| Received:October 22, 2003 |
| DOI： |
| KeyWords:Vascular endothelial growth factor Gene therapy Total-body irradiation Bone marrow microvasculature Repairing |
| FundProject:国家自然科学基金海外青年学者基金资助项目(39928010) |
| Author Name | Affiliation | E-mail | | ZHONG Zhao-dong | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | zouping@public.wh.hb.cn | | ZOU Ping | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | YOU Yong | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | 刘凌波 | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | 胡中波 | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | 郭荣 | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | 黄士昂 | | |
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| Abstract:: |
| Objective To explore the effect of vascular endothelial growth factor on the repairing of TBI- induced damage of bone marrow microvasculature in post-BMT mouse. Methods Mediated by adenovirus, hVEGF165 was expressed in TBI -conditioned BALB/c mice with syngeneic BMT. On +5 d, +10 d, +20 d, +30 d, the mice were injected with Chinese ink, following which the transparent tibias or plastic sections were used for analysis. Results hVEGF165 was successfully expressed by Ad-EGFP/hVEGF165. On +20 d, the diameter of central sinus in the VEGF group restored normally (P >0.05) and the percentage of microvasculature surface area returned to normal level (P >0.05). The percent cellularity of BM in the VEGF group began to be higher than that of other groups after +20 d (P <0.05). Conclusion The expression of hVEGF165 promotes the repairing of TBI -induced damage and the remodeling of bone marrow microvasculature, the resulting in rapid hematopoietic reconstruction. |
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