| LIU Li,LU Hai,CE.Ruebe,et al.Regulation of TNF-α level after irradiation of human NSCLC cell lines in vitro[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):313-316 |
| Regulation of TNF-α level after irradiation of human NSCLC cell lines in vitro |
| Received:December 16, 2003 |
| DOI： |
| KeyWords:Non-small cell lung cancer cell lines(A549,NCl-H596) Tumor necrosis factor(TNF-α) Ionizing radiation |
| FundProject:国家留学基金资助项目(20842007) |
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| Abstract:: |
| Objective Even though radiotherapy plays a major role in the local treatment of non-small cell lung cancer(NSCLC), little is known about the molecular effects of irradiation on this tumor.In the present study, we examined two NSCLC cell lines for their TNF-α production after irradiation. Methods Two human NSCLC cell lines(A549:squamous;NCl-H596:adenosquamous)were investigated for their TNF-α mRNA(real-time RT-PCR)and TNF-α protein expressions(ELISA) after exposure to different irradiation doses(2, 5, 10, 20, 30, 40 Gy) and time intervals(1, 3, 6, 12, 24, 48 or 72 h).The clonogenic survival was evaluated after irradiation with 2, 4, 6 and 8 Gy. Results Non-irradiated NSCLC cells exhibited no or very low TNF-α expression.For the NCl-H596 cell line, TNF-α expression was significantly elevated at 1-12 h(maximum 6 h:568-fold increase relative to unirradiated cells), and TNF-α protein production at 6-72 h post-irradiation(maximum 24 h:58-fold increase).The radiation-induced increase could already be observed after irradiation with 2 Gy.In contrast, A549 cells showed no irradiation-induced TNF-α production.The clonogenic survival of these cell lines was nearly indentical. Conclusion NCl-H596 cells produce significant quantities of TNF-α following irradiation in time-and dose-dependent manner.The pro-inflammatory cytokine TNF-α is a key mediator for the pathogenesis of radiation pneumonitis.Radiation-induced endogenous TNF-α expression in NSCLC cells may affect the normal lung tissue adjacent to the tumor and may be associated with an adverse clinical outcome of the patient. |
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