GUAN Xiao-xiang,CHEN Long-bang,YU Zheng-hong,et al.Cell cycle alteration and cell growth inhibition induced by 60Co γ-irradiation in HepG2 cell line[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):311-313
Cell cycle alteration and cell growth inhibition induced by 60Co γ-irradiation in HepG2 cell line
Received:January 26, 2004  
DOI:
KeyWords:HepG2  60Co  γ-ray  Cell cycle  p27kip1
FundProject:中国博士后科学基金资助项目(2003034383)
Author NameAffiliationE-mail
GUAN Xiao-xiang Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China 
longbangchen@yeah.com 
CHEN Long-bang Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China 
 
YU Zheng-hong Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China 
 
鹿红 Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China 
 
王靖华 Department of Oncology, General Hospital of Nanjing Military Command, Nanjing 210002
China 
 
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Abstract::
      Objective To study the cell cycle alterations in cells of human liver cancer cell line HepG2 in vitro after 60Co γ-irradiation; in order to investigate further the role of p27 kip1 protein on cell cycle and proliferation. Methods The effect on proliferation of HepG2 cell line by irradiation with 1.0-6.0 Gy γ-rays was evaluated with MTT assay. The cell cycle profile and apoptosis were analyzed by FACS, and the expression and subcellular localization of p27kip1 in the cells were detected by laser confocal microscopy. Results 60 Co γ-irradiation inhibited the growth of HepG2 cell line in a dose-dependent manner. The HepG2 cell line cells were arrested in G2/M 48 hours after 3.0 Gy γ-ray exposure. The expression of p27 kip1was down-regulated as compared to HepG2 cell line cells without γ- radiation exposure, Furthermore the p27kip1 was redistributed to cytoplasm after γ-irradiation. Conclusion The low expression and cytoplasmic localization of p27 kip1 might be due to the G2/M phase arrest induced by gamma- irradiation of HepG2 cell line cells.
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