| CHEN Jun,ZHANG Ji-ren,XIAO Ming-xing,et al.P-glycoprotein down-regulates the X-ray-induced apoptosis of a drug-resistant tumor cell line MCF-7/Adr[J].Chinese Journal of Radiological Medicine and Protection,2004,24(4):297-299 |
| P-glycoprotein down-regulates the X-ray-induced apoptosis of a drug-resistant tumor cell line MCF-7/Adr |
| Received:January 18, 2004 |
| DOI： |
| KeyWords:P-gp Mitochondrial membrane potenial Apoptosis X-ray |
| FundProject:国家自然科学基金资助项目(30100045) |
| Author Name | Affiliation | | CHEN Jun | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | ZHANG Ji-ren | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | XIAO Ming-xing | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | 余志坚 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China | | 陈春 | Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China |
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| Abstract:: |
| Objective To investigate the regulatory effect of P-gp on X-ray-induced apoptosis of drug-resistant tumor cells. Methods Anti-P-gp McAb was applied to block P-gp function. MCF-7/Adr, a P-gp-overexpressing drug-resistant breast cancer cell line; was irradiated with X-rays. Flow cytometry was performed to examine dynamic changes of apoptotic ratio and mitochondrial membrane potenial(Δ Ψm)at various times after X-ray irradiation. Results Apoptotic ratio of the P-gp-blocked group was 25.53%±2.85%;30.43%±2.21%;39.03%±2.60%, and of the control group was 16.13%±1.16%;21.73%±1.31%;27.53%±2.55% at 6 h, 12 h, 24 h, respectively, after X-ray irradiation.The apoptotic ratio of the P-gp-blocked group was significantly up-regulated, as compared with that of the control group( P <0.01). The mitochondrial membrane potenial(Δ Ψm) of both the P-gp-blocked group and the control group was decreased, but the Δ Ψm of the P-gp-blocked group was significantly lower than that of the control group (P < 0.01). Conclusion P-gp down-regulates the X-ray-induced apoptosis of the drug-resistant MCF-7/Adr cells and elevates the mitochondrial membrane potenial(Δ Ψm). |
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