| LI Yu,YANG Su-xia,ZHOU Shu-zhen,et al.Differentiation-inducting effect of Panshengan on BEL-7402 hepatoma cells[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):252-253 |
| Differentiation-inducting effect of Panshengan on BEL-7402 hepatoma cells |
| Received:January 09, 2004 |
| DOI： |
| KeyWords:Panshengan Hepatoma Differentiation induction |
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| Abstract:: |
| Objective To study differentiation-inducting effect of Panshengan on BEL-7402 hepatoma cells. Methods Using cell biology method to study the effect of Panshengan on growth characteristics of cells;morphometric method to study the effect on morphometric parameters of cells,nucleus and AgNORs,FACS to study the effect on cell cycle;conventional biochemical methods to study the effect on activities of ALP and LDH enzymes;and RNA dot blot to study the expression of c-N-ras. Results After exposure to Panshengan,the growth rate of cells was reduced apparently.The area,equivalent diameter,equivalent volume of cells and nucleus,and ratio of nucleus to plasma were decreased apparently with the increase of dosage by bearing good dose-effect relationship.In the mean time,the number and area of intranuclear AgNORs granules decreased remarkably.The cell cycle was detained at G0/G1 phase.The activities of two hepatoma signal enzymes,ALP and LDH,were reduced with relatively good dose-effect relationship.The expression of c-N-ras gene was suppressed. Conclusion It is proved preliminarily that Panshengan could induce differentiation of BEL-7402 hepatoma cells. |
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