| LI Ti-yuan,ZHANG Ying-nan,HUANG Rui-fang,China.Influence of 89Sr therapy with radiosensitizer on DNA damage of bone marrow in mice[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):247-248 |
| Influence of 89Sr therapy with radiosensitizer on DNA damage of bone marrow in mice |
| Received:December 26, 2003 |
| DOI： |
| KeyWords:DNA damage 89Sr Nicotinamide Carbogen Bone marrow Micronuclei |
| FundProject:广东省自然科学基金资助项目(000372) |
| Author Name | Affiliation | | LI Ti-yuan | Medical Research Center, Department of Nuclear Medicine, Shenzhen People's Hospital, Jinan University, Shenzhen, Guangdong | | ZHANG Ying-nan | 暨南大学医学院附属第二医院深圳市人民医院临床医学研究中心, 核医学科 | | HUANG Rui-fang | Medical Research Center, Department of Nuclear Medicine, Shenzhen People's Hospital, Jinan University, Shenzhen, Guangdong | | China | 暨南大学医学院附属第二医院深圳市人民医院临床医学研究中心, 核医学科 | | 郭一玲 | 深圳, 暨南大学医学院附属第二医院深圳市人民医院临床医学研究中心, 核医学科 | | 顾望为 | 第一军医大学实验动物中心 | | 林继红 | 第一军医大学实验动物中心 |
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| Abstract:: |
| Objective To study the influence of 89 Sr therapy with radiosensitizer on DNA damage of bone marrow in mice. Methods Chinese Kunming mice were divided into 5 groups respectively:negative control (saline),positive control ( 89 Sr), 89 Sr+nicotinamide, 89 Sr+Carbogen and 89 Sr+nicotinamide+carbogen groups. 89 SrCl was intravenously injected as a blous containing activites of 7400 kBq(200 μCi)200μl and saline was administered in equal volume in the negative control group.On days 1,2,3,4,6,8,15,20,30,60 and 90 after injection,mice were sacrificed for micronuclei assay in femoral marrow reticulocytes (MnRETs).One thousand randomly selected reticulocytes from each mouse were scored for the number of micronuclei. Results The average frequency of MnRETs induced as a function of time after injection is shown in a dual peak curve.The first maximum MnRETs frequency occurred between the 2nd and the 4th day,and the second between the 10th and the 14th day.A significant statistic difference on the MnRETs frequency was found between the negative and the positive control groups(P<0.001),while no differences were noted among 89 Sr+N+C, 89 Sr, 89 Sr+N and 89 Sr+C groups (P>0.05).There were also no significant differences on single,double or ternary micronuclei cell frequencies among 89 Sr+N+C, 89 Sr, 89 Sr+N and 89 Sr+C groups ( P>0.05).No tetrad microncuclei cell was found in the experiment. Conclusion Administration of radiosensitizer does not aggravate the toxicity on bone marrow. |
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