| ZHENG Hui,XUE Hong,ZHEN Rong.The changes in drug binding activity of GABA receptor and animal neural-behavior after gamma irradiation[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):243-245 |
| The changes in drug binding activity of GABA receptor and animal neural-behavior after gamma irradiation |
| Received:February 02, 2004 |
| DOI： |
| KeyWords:Gama-aminobutyric-acid receptor Gamma rays Equilibrium dissociation constant (K-d) Behavior Anxiety |
| FundProject:香港工业局科研基金资助(RGCHKUST613703M) |
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| Abstract:: |
| Objective The purpose of this study was to investigate the effect of irradiation on gama-aminobutyric-acid receptor (GABA-R) as well as behavioral changes after brain60 Co γ-irradiation. Methods The mice were irradiated with gamma rays (20 Gy;10 Gy and 5 Gy).The drug binding activity of GABA receptor in brain receptor was measured by fluorescence anisotropy (FA) and equilibrium dissociation constants.The behavioral changes were observed by the locomotor activity test,elevated plus-maze test and hole-board test at 1,10,24 and 48 hr after irradiation. Results 1.The drug binding activity of the GABA receptor was decreased and the equilibrium dissociation constant (Kd) was significantly increased compared with the negative control group 2 hr after irradiation,and a spike value appeared at 24 hr.It showed that the irradiation might damage or decrease the binding activity and the bio-activity of GABA receptor.2.The animal experiment confirmed that the irradiated animal model showed neural-behavioral changes of anxiety or depression.3.The decreased binding activity of GABA receptor and changes in behavior of irradiated animal were dependent on radiation intensity.4.The changes of behavior was similar to the blocked GABA receptor group.It suggests the relationship of radiation and GABA receptor. Conclusion These results suggest that GABA receptor may be involved in radiation injury.The functional changes of GABA receptor may be an induction factor of behavioral disorder.The article also discussed the effect of anxiety and results obtained from the point of view of GABA receptor system involvement in the changes observed after irradiation. |
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