| XIE Liang-xi,LI De-rui,LIN Ying-cheng.Arsenic trioxide enhances radiosensitivity of nasopharyngeal carcinoma in vitro[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):240-242 |
| Arsenic trioxide enhances radiosensitivity of nasopharyngeal carcinoma in vitro |
| Received:May 18, 2003 |
| DOI： |
| KeyWords:Nasopharyngeal neoplasm Radiotherapy Arsenic trioxide Radiosensitizer |
| FundProject:广东省中医药局科研基金资助项目(102053); 汕头大学研究和发展基金项目(L03002) |
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| Abstract:: |
| Objective To evaluate the ability of arsenic trioxide to sensitize nasopharyngeal carcinoma(NPC) to radiation in vitro . Methods CNE-1 human nasopharyngeal cancer cell line was treated with arsenic trioxide.Cell survival was determined by clonogenic assay.Cell cycle distribution after As2O3 treatment was analyzed by using flow cytometry.Immunochemistry was used to determine bax,p53 and bcl-2 expressions. Results As2O3 definitely enhanced radiosensitivity of CEN-1 cells.Cell survival experiments showed sensitivity-enhancement ratio(SER) of 1.33 and 1.57 for 1.0 μmolL and 1.5 μmolL of As2O3,respectively.DNA flow cytometric analysis indicated that As2O3(0.5～2.0 μmolL) induced efficiently G-2M phase arrest in this cell line following 48 hours of exposure.Meanwhile,the expression of p53 and baxbcl-2 increased after As2O3 treatment. Conclusion As2O3 might be a potential radiosensitizer for NPC. |
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