| LIU Long,LIU Lu,TONG Guan-sheng.Study on apoptosis of human non-small cell pulmonary carcinoma A549 cells induced by 32P-chromium phosphates in vitro[J].Chinese Journal of Radiological Medicine and Protection,2004,24(3):234-236 |
| Study on apoptosis of human non-small cell pulmonary carcinoma A549 cells induced by 32P-chromium phosphates in vitro |
| Received:January 15, 2004 |
| DOI： |
| KeyWords:Tumor Non-small cell lung cancer Apoptosis Radioactive nucleotied 32P chromium phosphate |
| FundProject:江苏省科委应用基础基金资助项目(BJ2000004); 江苏省卫生厅科研基金资助项目(H200117) |
| Author Name | Affiliation | E-mail | | LIU Long | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | luliuzhou.@gina.com | | LIU Lu | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | | | TONG Guan-sheng | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | | | 封国生 | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | | | 高宏 | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | | | 高文 | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | | | 李澄 | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | | | 刘志勇 | Institute of Nuclear Medicine Technology of Southeast Unviersity, Nanjing 210009, China | |
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| Abstract:: |
| Objective To observe the phasic change and apoptosis of A549 non-small cell lung cancer cells induced by 32 P-chromium phosphate in vitro ,and establish the dose-response and time-response relationship. Methods Internal irradiation was conducted by adding 32 P colloid into A549 cell culture system.The initial radioactivities were 0,93,180,278,370,463 MBqL,respectively.Giemsa stain,transmission electron microscopy and TUNEL were used in assessing morphologic,ultra structural pathologic and biochemical characteristics,and the phasic changes and apoptotic rates of cells were studied by flow cytometry. Results After irradiation of A549 cells,the cell ratio of S+G2-M phase tended to increase within 96 h,then decreased gradually.At 72 h after irradiation the A549 cells showed excited manifestation,and in each irradiation group apoptosis began from 96 h of irradiation,and attained the peak at 120 h. Conclusion In the lower dosagerange, 32 P internal irradiation may induce human NSCLC A549 cells to present delayed onset of apoptosis,and the rate of cell apoptosis is positively correlated to the initial radioactivity concentration. |
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